Ampicillin injection for veterinary use is a potent antibiotic employed in dogs, cats, cattle, and calves to combat bacterial infections. With broad-spectrum activity against gram-positive and gram-negative bacteria, it is utilised for respiratory, urinary, gastrointestinal, and skin infections. Administered subcutaneously or intramuscularly, proper dosing is crucial based on infection type and animal weight. By inhibiting bacterial cell wall synthesis, ampicillin effectively eradicates susceptible bacteria. While generally well-tolerated, side effects like gastrointestinal disturbances may occur. Understanding its indications, administration, and precautions ensures effective treatment and contributes to the health and well-being of animals under veterinary care.